MIQE: A Step Toward More Robust and Reproducible Quantitative PCR

MIQE: A Step Toward More Robust and Reproducible Quantitative PCR.

Featured Article: Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, et al. The MIQE guidelines: minimum information for publication of quantitative realtime PCR experiments. Clin Chem 2009;55:611–22. The concept of using in vitro enzymatic synthesis to amplify DNA was first mooted in 1971 (1 ) and demonstrated in 1985 as the “polymerase chain reaction” (2 ). PCR enables the dete...

Real-time quantitative PCR, pathogen detection and MIQE.

Nucleic acids are the ultimate biomarker and real-time PCR (qPCR) is firmly established as the method of choice for nucleic acid detection. Together, they allow the accurate, sensitive and specific identification of pathogens, and the use of qPCR has become routine in diagnostic laboratories. The reliability of qPCR-based assays relies on a combination of optimal sample selection, assay design ...

Modifying “Pico” Question into “Picos” Model for More Robust and Reproducible Presentation of the Methodology Employed in A Scientific Study

Enabling reproducible real-time quantitative PCR research: the RDML package

Motivation Reproducibility, a cornerstone of research, requires defined data formats, which include the setup and output of experiments. The real-time PCR data markup language (RDML) is a recommended standard of the minimum information for publication of quantitative real-time PCR experiments guidelines. Despite the popularity of the RDML format for analysis of quantitative PCR data, handling o...

The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments.

BACKGROUND Currently, a lack of consensus exists on how best to perform and interpret quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of sufficient experimental detail in many publications, which impedes a reader's ability to evaluate critically the quality of the results presented or to repeat the experiments. CONTENT The Minimum Information for Publicatio...